mir-26b enhances radiosensitivity of hepatocellular carcinoma cells by targeting epha2
نویسندگان
چکیده
objective(s): although low-dose radiotherapy (rt) that involves low collateral damage is more suitable for hepatocellular carcinoma (hcc) than traditional high-dose rt, but to achieve satisfactory therapeutic effect with low-dose rt, it is necessary to sensitize hcc cells to irradiation. this study was aimed to determine whether radiosensitivity of hcc cells can be enhanced using mir-26b by targeting erythropoietin producing human hepatocelluar a2 (epha2). materials and methods: the levels of mir-26b and epha2 expression in multiple hcc cell lines were assessed by qpcr and western blotting, respectively, and compared with those in a hepatic cell line. hcc 97h cells were transfected with mir-26b mimics, epha2-shrna or epha2 over-expression vector before exposure to low-dose irradiation. results:different degrees of mir-26b down-regulation and epha2 up-regulation were observed in all hcc cell lines, among which the hcc 97h cell line expressed the lowest level of mir-26b and highest level of epha2. epha2 was verified as the target of mir-26b by dual luciferase reporter assay. hcc 97h cells transfected with mir-26b mimics or epha2-shrna reduced the expression of epha2 protein, with significantly lower cell proliferation rate and cell invasion ability and higher apoptosis rate in response to low-dose irradiation than those in the non-transfected cells. these results were reversed after epha2 was overexpressed by transfection with the epha2 overexpression vector. co-transfection with mir-26b mimics and epha2 overexpression vector barely altered epha2 expression level and cell response to low-dose irradiation. conclusion: these data suggest that mir-26b enhances radiosensitivity of hcc 97h cells by targeting epha2 protein.
منابع مشابه
miR-26b enhances radiosensitivity of hepatocellular carcinoma cells by targeting EphA2
Objective(s): Although low-dose radiotherapy (RT) that involves low collateral damage is more suitable for hepatocellular carcinoma (HCC) than traditional high-dose RT, but to achieve satisfactory therapeutic effect with low-dose RT, it is necessary to sensitize HCC cells to irradiation. This study was aimed to determine whether radiosensitivity of HCC cells can be enhanced using miR-26b by tar...
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عنوان ژورنال:
iranian journal of basic medical sciencesجلد ۱۹، شماره ۸، صفحات ۸۵۱-۸۵۷
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